Coding

Part:BBa_K1773005:Design

Designed by: Sarunas Tumas   Group: iGEM15_Vilnius-Lithuania   (2015-06-30)

I-F type CRISPR-Cas Cascade complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1790
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2307


Design Notes

Mutagenesis primers:

Fw: cgcacatttcccgtggaatccactcttcctcaaaagg

Rev: ccttttgaggaagagtggattccacgggaaatgtgcg


Primers for Suffix and prefix insertion:

Fw: actgaattcgcggccgcttctagatgataactaaaacaacagtagtaaaagc

Rev: cggctgcagcggccgctactagtaattaattagaaatgtggaacggttg



Source

Aggregatibacter actinomycetemcomitans D-7S

References